Coding

Part:BBa_K3512001:Design

Designed by: Gourav Saha   Group: iGEM20_BITSPilani-Goa_India   (2020-10-20)


ccdB Toxin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 216


Design Notes

The toxin is part of the toxin-antitoxin system created to be part of the killswitch for iGEM BITS Goa's project. It is placed under a modified inducible system using the FNR promoter.


Source

The toxin-antitoxin system is found in the F plasmid in bacteria.

References